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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Virus Res. 2019 Jun 28;270:197646. doi: 10.1016/j.virusres.2019.197646

Figure 3. The Golgi Sorting Motifs of UL138 are not required for UL138-mediated suppression of IE1 transcript and protein accumulation in HCMV-infected THP-1 cells.

Figure 3

A. IE transcripts from THP-1 cells infected with WT AD169 (AD) or UL138 expressing recombinant AD169 (AD138) or the UL138 quadruple Golgi mutant recombinant AD169 (AD138Δ4) at an MOI of one for 18 h in the presence (+) or absence (−) of VPA were quantitated by qRT-PCR. Data represent the mean ± SEM of three independent biological replicates. * p<0.05; ns, not significant (p>0.05) by Student’s t test. B. Lysates from THP-1 cells infected with the indicated viruses at an MOI of one for 18 h were analyzed for viral genomes by qPCR. Results are plotted relative to WT virus infection. Data represent the mean ± SEM of three independent biological replicates. ns, not significant (p>0.05) by Student’s t test. C. Lysates from THP-1 cells infected with the indicated viruses at an MOI of one for 18 h in the presence (+) or absence (−) of VPA were analyzed by western blot with the indicated antibodies. Representative images of three independent biological replicates are shown. D. Protein from experiments in panel C was quantified by densitometry and compared relative to WT AD169 (AD) infection. Data represent the mean ± SEM of three independent biological replicates. *** p<0.001; * p<0.05 by Student’s t test. E. Lysates from fibroblasts infected with the indicated virus at an MOI of one harvested at the indicated time point post infection were analyzed by western blot with the indicated antibodies. Representative images of three independent biological replicates are shown.