Fig. 1: Experimental design.
(a) Female C57BL/6J mice were exposed to cefoperazone ad libitum in drinking water for days −7 to −2 prior to inoculation with 1 × 106 spores of the C. difficile strain M7404 by gavage on day 0. Following infection mice were administered intraperitoneal injections of misoprostol once daily for 7 days. (b) Mice were exposed to cefoperazone as in (a) then treated with misoprostol (20 μg) daily by intraperitoneal injection through 10 days. Feces were collected for microbiota studies.
