KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-paxillin | BD Biosciences | cat. no. 610568; RRID: AB_397917 |
| anti-phospho-paxillin (Y118) | Thermo Fisher | cat. no. 44–722G; RRID: AB_2533733 |
| anti-phosphotyrosine (4G10) | Millipore | cat. no. 05–321; RRID: AB 309678 |
| anti-cortactin (p80/85) clone 4F11 | Millipore | cat. no. 05–180 RRID: AB_309647 |
| anti-phospho-cortactin (Y466) | Abeam | cat. no. ab51073 RRID: AB_869231 |
| anti-p130Cas | BD Biosciences | cat. no. 610272 RRID: AB_397667 |
| anti-phospho-p130Cas (Y249) | BD Biosciences | cat. no. 558401 RRID: AB 647286 |
| anti-SRC | Cell Signaling | cat. no. 2108; RRID: AB_10695298 |
| anti-SRC | Santa Cruz | cat. no. 8056; RRID: AB_627306 |
| anti-phospho-SRC family (Y416) | Cell Signaling | cat. no. D49G4; RRID: AB_10013641 |
| anti-VE cadherin | Santa Cruz | cat. no. sc-6458; RRID: AB_2077955 |
| anti-phospho-VE cadherin (Y658) | Thermo Fisher | cat. no. 44–1144G; RRID: AB_2533583 |
| anti-phospho-VE cadherin (Y731) | Thermo Fisher | cat. no. 44–1145G; RRID: AB_2533584 |
| anti-phospho-VE cadherin (Y685) | gift from the laboratory of Dr. Elisabetta Dejana (IFOM) | Orsenigo et al., 2012 |
| anti-β-catenin | Santa Cruz | cat. no. 1496; RRID: AB_1249372 |
| anti-P120-catenin | Santa Cruz | cat. no. sc-1101; RRID: AB_632091 |
| anti-GAPDH | Ambion | cat. no. AM4300; RRID: AB_437392 |
| anti-mCherry | Biovision | cat. no. 5993–100; RRID: AB_1975001 |
| anti-GFP | Clontech | cat. no. 63238; 1 RRID: AB_2313808 |
| Bacterial and Virus Strains | ||
| RapR-SRC-as2-cerulean-myc Adenovirus | Klomp et al., 2016 | |
| RapR-SRC-cerulean-myc Adenovirus | This Paper | N/A |
| RapR-SRC-R175L-cerulean-myc Adenovirus | This Paper | N/A |
| RapR-LYN-cerulean-myc Adenovirus | This Paper | N/A |
| RapR-SRC-as2-mCherry-myc Adenovirus | This Paper | N/A |
| mCherry-FRB Adenovirus | This Paper | N/A |
| GFP(Y66S)-FRB Adenovirus | This Paper | N/A |
| VE cadherin-GFP Adenovirus | Dr. William Muller (Northwestern University) | Gonzalez et al., 2016 |
| VE cadherin-GFP Y658F Adenovirus | Dr. William Muller (Northwestern University) | Gonzalez et al., 2016 |
| VE cadherin-GFP Y731F Adenovirus | Dr. William Muller (Northwestern University) | Gonzalez et al., 2016 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| D-erythro-sphingosine-1-phosphate (CAS 26993–30–6) | Avanti Polar Lipids | cat. no. 860492 |
| Human Alpha Thrombin (Factor lla) (CAS 9002–04–4) | Enzyme Research Laboratories | cat. no. HT1002a |
| Fluorescein isothiocyanate-avidin | Invitrogen | cat. no. 43–4411 |
| normal donkey serum | Jackson ImmunoResearch | cat. no. 017–000–121 |
| Cy™5 anti-rabbit | Jackson ImmunoResearch Laboratories | cat. no. 711–175–152 RRID: AB_2340607 |
| Alexa Fluor® 488 anti-goat | Jackson ImmunoResearch Laboratories | cat. no. 705–545–003 RRID: AB_2340428 |
| Alexa Fluor® 647 anti-goat | Jackson ImmunoResearch Laboratories | cat. no 705–607–003 RRID: AB_2340439 |
| Alexa Fluor® 647 anti-mouse | Jackson ImmunoResearch Laboratories | cat. no 715–605–151 RRID: AB_2340863 |
| Rapamycin (CAS 53123–88–9) | LC Laboratories | cat. no. R5000 |
| Saracatinib (CAS 379231–04–6) | Santa Cruz | cat. no. sc-364607 |
| 1-Naphthyl-PP1 (CAS 221243–82–9) | Cayman | cat. no. 10954 |
| Fluorescein isothiocyanate-dextra | Sigma-Aldrich | cat. no. 46944 |
| Gelatin | Sigma-Aldrich | cat. no. G2500 |
| Phalloidin Alexa Fluor™ 647 | Thermo Fisher | cat. no. A22287 RRID: AB_2620155 |
| Experimental Models: Cell Lines | ||
| HPAEC-Human Pulmonary Artery Endothelial cells - Passage 6–8 |
Lonza | cat. no. CC-2530 |
| HUVEC-Human Umbilical Vein Endothelial cells - Passage 6–8 |
Lonza | cat. no. C2517A |
| Oligonucleotides | ||
| Notl-XFP FWD 5’- atatatGCGGCCGCatggtgagcaagggcgagga −3’ | This Paper | N/A |
| FRB-EcoRV REV 5’- atatatGATATCttaactagtctttgagattc −3’ | This Paper | N/A |
| Recombinant DNA | ||
| RapR-LYN-cerulean-myc plasmid | This Paper | N/A |
| RapR-SRC-cerulean-myc plasmid | Karginov et al., 2014 | |
| RapR-SRC-as2-cerulean-myc plasmid | Klomp et al., 2016 | |
| RapR-SRC-as2-mCherry-myc plasmid | Klomp et al., 2016 | |
| RapR-SRC-R175L-cerulean-myc plasmid | Karginov et al., 2014 | |
| mCherry-FRB plasmid | Karginov et al., 2014 | |
| Y66S-GFP-FRB plasmid | This Paper | |
| pShuttle vector | Addgene | Plasmid no. 16402 |
| mEos2-Paxillin-22 | Addgene | Plasmid no. 57409 |
| Stargazin-mVenus | Klomp et al., 2016 | |
| Software and Algorithms | ||
| Metamorph Image Analysis Software | https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy#gref | |
| Imaris Image Analysis Software | http://www.bitplane.com/ | |
| GraphPad Prism Software | www.graphpad.com | |
| FIJI - ImageJ | ||
| CellGeo software | Schindelin et al., 2012 | |
| MovThresh software | Tsygankov et al., 2014 | |
| ProActive software | Tsygankov et al., 2014 | |
| PeakSelector | Sage et al., 2015 | |