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. Author manuscript; available in PMC: 2020 Aug 15.
Published in final edited form as: Cell Chem Biol. 2019 May 23;26(8):1180–1186.e5. doi: 10.1016/j.chembiol.2019.04.008

Figure 4. Modulation of the hypoxic circuit through selective inhibition of miR-210 causes apoptosis in hypoxic MDA-MB-231 cells.

Figure 4.

(A) RT-qPCR analysis of validated miRNAs indicated miR-210 as the most significantly downregulated miR upon TGP-210-RL (200 nM) treatment. (B) Modulation of GPD1L and HIF1a transcripts with TGP-210-RL (200 nM) treatment after 24 h and 48 h, as determined by RT-qPCR. (C) Apoptosis in hypoxic MDA-MB-231 cells treated with miR-210-targeted locked nucleic acid (LNA-210), scramble locked nucleic acid (Scr-LNA), TGP-210, or TGP-210-RL, as measured by Caspase 3/7 activity. Cells that overexpress pre-miR-210 via a plasmid are shown in the yellow box. *p < 0.05, **p < 0.01, as determined by a two-tailed Student t test. Data represent means ± SEM of biological triplicates. See Figure S3 and Table S2.