KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-PDCD4 | Santa Cruz Biotechnology | sc-376430 |
| Mouse anti-PTEN | Santa Cruz Biotechnology | sc-7974 |
| rabbit anti-GAPDH | Cell Signaling Technology | 2118 |
| goat anti-mouse IRDye 800CW | LI-COR Biotechnology | 926–32210 |
| goat anti-rabbit IRDye 680RD | LI-COR Biotechnology | 926–68071 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Butylcycloheptyl prodiginine (bPGN) | Developmental Therapeutics Program (DTP) of the National Cancer Institute (NCI). | -N/A- |
| 5-Fluorouracil | Sigma | F6627 |
| obatoclax | selleckchem | S1057 |
| Prodigiosin hydrochloride | Sigma | P0103–100UG |
| Navitoclax | selleckchem | S1001 |
| spermidine | Sigma | S2626–1G |
| Methoctramine hydrate | Sigma | M105–10MG |
| Hexachlorophene | selleckchem | S4632 |
| Regorafenib | selleckchem | S1178 |
| SYBR Green Dye | ThermoFisher | S7567 |
| Critical Commercial Assays | ||
| XTT | ThermoFisher | X6493 |
| mirVana PARIS | ThermoFisher | AM1556 |
| Dicer reaction assays | Genlantis | T510001 |
| NanoString nCounter Human Pan-Cancer Pathway Panel assay kit | nanoString Technology | XT-CSO-PATH1–12 |
| Deposited Data | ||
| NanoString_Matarlo_mRNA Time point studies of bPGN treated HCT-116 cell line |
This paper |
GSM3731921 GSM3731922 GSM3731923 GSM3731924 GSM3731925 GSM3731926 |
| Experimental Models: Cell Lines | ||
| HCT-116 colorectal cancer cell line, human | ATCC | ATCC CCL-247 |
| CoN - Normal colon cell line, human | ATCC | ATCC CRL-1790 |
| Oligonucleotides | ||
| pre-miR-21, UGUCGGGUAGCUUAUCAGACUGAUGUUGACUGUUGAAUCUCAUGGCAACACCAGUCGAUGGGCUGUCUGACA | Eurofins Scientific | https://www.eurofinsgenomics.com/en/products/products.aspx |
| miR-21, UAGCUUAUCAGACUGAUGUUGA | Eurofins Scientific | https://www.eurofinsgenomics.com/en/products/products.aspx |
| HIV TAR, CUGGUUAGACCAGAUCUGAGCCUGGGAGCUCUCUGGCUAACUAG | Eurofins Scientific | https://www.eurofinsgenomics.com/en/products/products.aspx |
| Pre-miR-638, GUGAGCGGGCGCGGCAGGGAUCGCGGGCGGGUGGCGGCCUAGGGCGCGGAGGGCGGACCGGGAAUGGCGCGCCGUGCGCCGCCGGCGUAACUGCGGCGCU | Eurofins Scientific | https://www.eurofinsgenomics.com/en/products/products.aspx |
| Lys3 tRNA, GCCCGGAUAGCUCAGUCGGUAGAGCAUCAGACUUUUAAUCUGAGGGUCCAGGGUUCAAGUCCCUGUUCGGGCGCCA | Miller JT et al., 2004 | N/A |
| miR-21 siRNA mimic | ThermoFisher | 4464066 |
| miR-21 siRNA inhibitor | ThermoFisher | 4464084 |
| qPCR primers, pre-miR-21: forward – TGTCGGGTAGCTTATCAGAC, reverse – TTCAGACAGCCCATCGACTG PDCD4: forward – TAGATTGTGTGCAGGCTA, reverse – GTTCAGCTTCAGATATGTCTC PTEN: default Exiqon construct GAPDH: default Exiqon construct |
EXIQON ExiLERATE | 303402 |
| Software and Algorithms | ||
| GraphPad Prism 7.03 | Graphpad (NIH) | https://www.graphpad.com/scientific-software/prism/ |
| FIJI | NIH | N/A |
| Imaris | Bitplane | N/A |
| NanoString nSolver | nanoString Technologies | https://www.nanostring.com/products/analysis-software/nsolver |
| nSolver Pathway Analysis; Advanced Analysis | nanoString Technologies | https://www.nanostring.com/products/analysis-software/advanced-analysis |