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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Biol Psychiatry. 2019 Apr 3;86(5):377–387. doi: 10.1016/j.biopsych.2019.03.982

Figure 6: Stimulating MMPs with tissue plasminogen activator (tPA) or integrins with RGD peptide potentiated cue-induced cocaine seeking.

Figure 6:

(A) Time course of active and inactive lever pressing for self-administration and extinction in cocaine-treated rats for tPA experiment. (B) Location of microinjection cannula tips in the NAcore for all rats used in figure 6C. Numbers refer to mm to bregma. (C) tPA bilateral microinjection into NAcore potentiate cue-induced cocaine seeking (2-way ANOVA, repeated measures over extinction/reinstatement, Veh/tPA/β3/FAKi F(3,22)=10.05, p<0.001; EXT/RST F(1,22)=40.66, p<0.001; interaction F(3,22)=6.99, p=0.002) and β3 Integrin or FAKi microinjections in NAcore prevented tPA induced potentiation in cued-cocaine seeking. No statistical difference was observed in inactive lever presses during cued-induced cocaine-seeking after Veh, tPA, β3 or FAKi microinjections in NAcore. (D) Time course of active and inactive lever pressing for self-administration and extinction in cocaine-treated rats for RAD/RGD experiment. (E) Location of microinjection cannula tips in the NAcore for rats used in figure 6F. (F) RGD (10 nmol) microinjection in NAcore potentiate cue-induced reinstatement in cocaine trained rats (RAD/RGD F(1,18)=4.52, p=0.047; EXT/RST F(1,18)=47.31, p<0.001; interaction F(1,18)=4.81, p=0.042). No statistical difference was observed in inactive lever presses during cued-induced cocaine-seeking after RAD or RGD microinjections in NAcore.

+p<0.05 compared to EXT using a Bonferroni’s post hoc test

*p<0.05 compared to VEH or RAD using a Bonferroni’s post hoc test

#p<0.05 compared to tPA treatment in panel C using a Bonferroni’s post hoc test