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(A) Schematic of SrtA-mediated reversible stiffening. (B) Effect of incubation time on stiffening of PEG-peptide hydrogels (2.5 wt% PEGNB, 25 μM SrtA). (C) Effect of SrtA concentration on stiffening of PEG-peptide hydrogels (2.5 wt% PEGNB, 4 hr incubation time). (D) Effect of glycine concentration on softening of PEG-peptide hydrogels (2.5 wt% PEGNB, 25 μM SrtA). (E) SrtA-mediated hydrolytic degradation of PEG-peptide hydrogels (2.5 wt% PEGNB, 16 hour incubation).
