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. 2019 Aug 12;12:6361–6370. doi: 10.2147/OTT.S210800

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© 2019 Wei et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Silencing MTHFD2 in CRC cells HCT116 and CACO-2 inhibited the proliferation and migration of cells. (A) HCT116 and caco-2 were transfected with si1- MTHFD2 and si2-MTHFD2, respectively. Silencing efficacy was detected with Western blot. (B) MTHFD2 mRNA and protein expression levels were measured in HCT116 and CACO-2 cells after transfection with si1-MTHFD2. (C) Colony-forming experiment showed the cell proliferative capacity of HCT116 and CACO-2 after transfection with si1-MTHFD2. (D) MTT assay analysis showed the cell growth of HCT116 and CACO-2 after transfection with si1-MTHFD2. (E) Transwell cell migration assay showed the cell migratory ability of HCT116 and CACO-2 after transfection with si1-MTHFD2. (**P<0.01, ***P<0.001, compared to the control group).

Abbreviations: MTHFD2, Methylenetetrahydrofolate dehydrogenase 2; CRC, colorectal cancer.