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. 2019 Aug 16;10:3698. doi: 10.1038/s41467-019-11627-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — HPLC chromatograms of reactions catalyzed by Spn-LicC and Ari-PntC (Ari1348). a Time-resolved reaction of CTP (open circle) with: I, P-Cho and Spn-LicC to make CDP-Cho (filled circle), and II, AEP and Ari-PntC to generate CMP-AEP (open square). b Spn-LicC more readily produces CDP-Cho (panel II) than CMP-AEP (panel III); in contrast, Ari-PntC preferentially generates CMP-AEP (panel IV) compared to CDP-Cho (panel V) after 30 min of reaction time. All reactions contained 50 nM enzyme, 1 mM of either AEP or P-Cho, 4 mM CTP, and 7 mM MgCl₂ in 50 mM Tris–Cl, pH 8.0