Fig. 2.

CD20^dim CD4⁺ T cells are enriched in HIV-1 RNA. Unstimulated PBMCs were subjected to the RNA FISH-flow protocol to detect HIV RNA expression in CD20^dim CD4⁺ T cells. a Gating strategy used to determine the HIV RNA expression on CD20⁻ and CD20^dim CD4⁺ T cells and expression of CD20 in HIV RNA⁺ cells. Previous sequential gates are represented in Supplementary Fig. 9. b Frequency comparison of HIV RNA⁺ cells in CD20^dim CD4⁺ T cells and CD20⁻ CD4⁺ T cells of ART-suppressed and viremic patients. Wilcoxon test was used. c Mean distribution of HIV RNA⁺ cells in the CD20⁺ and CD20⁻ phenotypes. Median values and min and max ranks are represented in panels (b) and (c). In all panels, n = 12 ART-suppressed and n = 11 viremic patients are shown. Data from patients #15–26, 60, 63, and 73–81 are included. Data underlying this Figure are provided as Source Data file