Table 1.
Parameter | Control | Control-level FeS | FeS-OE |
---|---|---|---|
Relative chlorophyll (SPAD) | 50.79 ± 1.60 | 51.56 ± 1.61 | 51.78 ± 2.06 |
Leaf thickness, mm | 0.76 ± 0.17 | 0.74 ± 0.12 | 0.72 ± 0.11 |
φPSII | 0.598 ± 0.010 | 0.597 ± 0.004 | 0.624 ± 0.004* |
φNPQ | 0.205 ± 0.011 | 0.200 ± 0.005 | 0.182 ± 0.003* |
φNO | 0.197 ± 0.002 | 0.201 ± 0.001 | 0.194 ± 0.002 |
Chlorophyll (a+b), g m−2 | 0.39 ± 0.06 | 0.38 ± 0.05 | |
Chlorophyll a/b | 5.05 ± 0.13 | 5.10 ± 0.10 | |
Rubisco, g m−2 | 0.35 ± 0.03 | 0.34 ± 0.02 |
“Control-level FeS”, T2 plants of the line 230(4)-7 with the control plants-levels of Rieske FeS protein; “FeS-OE”, T2 plants of the line 230(4)-7 with higher abundance of Rieske FeS per leaf area. MultispeQ measurements were performed at the incident irradiance of 200 µmol m−2 s−1, mean ± SE, n = 16 (4 biological replicates): φPSII quantum yield of PSII, φNPQ quantum yield of non-photochemical quenching, φNO quantum yield of non-regulatory energy dissipation, SPAD Soil Plant Analysis Development chlorophyll meter. Chlorophyll and Rubisco measurements are mean ± SE, n = 3 biological replicates. Asterisks indicate statistically significant differences between transgenic and control plants (P < 0.05). Zero(Null)-segregates were used as control plants