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In silico docking analysis of FluA DH M1 with the natural PPant-substrate intermediate 4. Substrate 4 interacts with all three DH substrate-binding regions a. b A close inspection of the dipeptide moiety of 4 shows close interactions with residues located between α3 and β11 (light blue) and α2 and β8 (dark blue). c A 90˚ horizontal rotation of a and close-up of the substrate 4 and interacting residues (colour figure online)
