Fig. 1.

a HPLC-chromatogram of a recombinantly produced protein measured directly from the supernatant at harvest (1), the same sample but pre-purified via Protein A (2) and the adalimumab reference (3), using the pH 7 buffer system. b Amount of IgG as a function of the integrated total peak area for the shaker data [grey-filled circle] and control standards [filled triangle]. c Amount of host cell proteins (HCPs) and IgG of fed-batch experiments at 37 °C [filled hexagon], 34 °C [unfilled hexagon] and 31 °C [grey hexagon]. d CEX chromatogram of a mocking supernatant (1), a standard spiked into the same supernatant (2) and the standard (3). K0 main variant, K1 1-lysine variant, K2 2-lysine variant, A1 acidic variant without further characterization. Linear regression was performed on the adalimumab dataset