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. 2019 Jun 7;46(8):1167–1178. doi: 10.1007/s10295-019-02202-5

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Fed-batch process parameters: a VCC, b protein titer, c lactate and d glucose concentration and e osmolality as a function of process time. f Specific glucose consumption (q_gluc) as a function of the growth rate (µ). 37 °C + Feed 1 [filled square], 37 °C + Feed 2 [filled diamond], 37 °C + Feed 3 [filled hexagon], 34 °C + Feed 1 [unfilled square], 34 °C + Feed 2 [unfilled diamond], 34 °C + Feed 3 [unfilled hexagon], 31 °C + Feed 1 [grey square], 31 °C + Feed 2 [grey diamond], 31 °C + Feed 3 [grey hexagon]. Fed-batch process started at day 3. Vertical dashed line indicates temperature shift (≈ day 4). Short dashed lines indicate trends