Fig. 6.

C14:0 is a competitive human TLR4 antagonist. HEK293 cells were transfected with plasmids encoding hTLR4, hMD2, and hCD14 together with a luciferase reporter plasmid dependent on NF-κB activation and a constitutively active reporter vector-encoding renilla luciferase. Two days after transfection, cells were incubated for 1 h with medium alone or the indicated amount of CLs, then washed and incubated 6 h with increasing amount of LPS. Luciferase and renilla were then quantified in cell lysate, and normalized and reported here as fold induction as compared to control. Each bar represents the mean ± standard deviation of three biological replicates (n = 3). Graphs are representative of at least three independent experiments