Fig. 8.

Shorter and more unsaturated CLs are more potent human TLR4 antagonists. HEK293 cells were transfected with plasmids encoding hTLR4, hMD2, and hCD14 together with a luciferase reporter plasmid dependent on NF-κB activation and a constitutively active reporter vector-encoding renilla luciferase. Two days after transfection, cells were incubated for 1 h with increasing amount of CLs, and then washed and incubated 6 h with the indicated amount of LPS. Luciferase and Renilla were then quantified in cell lysate, and normalized and reported here as percentage of the value measured for LPS alone. Each bar represents the mean ± standard deviation of three biological replicates (n = 3). Graphs are representative of at least three independent experiments. For C14:0, it was only possible to plot three points due to problems of solubility and cell viability