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. 2019 Aug 16;10:3701. doi: 10.1038/s41467-019-11686-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Arabidopsis MNS3 is a Golgi-resident protein. a The early steps of N-glycan processing in A. thaliana are shown. The three branches of the N-linked Glc₃Man₉GlcNAc₂ oligosaccharide precursor are marked A, B, and C. The red circle indicates the terminal α1,2-linked mannose residue that is cleaved off by MNS3 from the B-branch. GCSI: α-glucosidase I; GCSII: α-glucosidase II; MNS3: ER-α-mannosidase I; MNS1/MNS2: Golgi-α-mannosidase I. b Illustration of the domain organization of MNS3. C, cytoplasmic tail (41 aa); T, predicted transmembrane domain (26 aa); S, stem region (42 aa); CD, catalytic domain (515 aa). Positions of putative N-glycosylation sites are indicated (Y). The depicted numbers represent the length of the protein (624 amino acids). c Subcellular localization of MNS3. Confocal images show N. benthamiana leaf epidermal cells transiently expressing MNS3-GFP (green) alone (scale bar = 20 µm) and in combination with the cis/medial-Golgi protein MNS1-mRFP (magenta, scale bar = 5 µm) or the medial/trans-Golgi marker STtmd-mRFP (magenta, scale bar = 5 µm). The insets show a higher magnification of individual dual-colored Golgi stacks (scale bar = 2 µm). Images were acquired 2 days post infiltration (dpi)