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. 2019 Aug 16;10:3701. doi: 10.1038/s41467-019-11686-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Distinct cis-Golgi membrane proteins respond differently to BFA. Fluorescent protein fusions were transiently expressed in N. benthamiana leaf epidermal cells and observed 2 dpi on a confocal microscope. Images show the subcellular localization of (a) MNS3-GFP, (b) the Qa-SNARE GFP-AtSYP31, (c) the golgin GFP-AtCASP, and (d) the K/HDEL recycling receptor AtERD2-GFP before and after 1 h BFA treatment. Scale bars = 10 µm