Fig. 6.

The MNS3-CTS region contains signals that overrule HDEL-mediated ER retrieval. a Schematic illustration depicting the domain structure of the protein fusions MNS1-MNS3-GFP-HDEL, containing the MNS1-CTS region and MNS3 catalytic domain (CD), and MNS3-MNS1-GFP-HDEL, containing the MNS3-CTS region and MNS1 catalytic domain, both C-terminally tagged with GFP and the ER retrieval signal HDEL. b Confocal images showing N. benthamiana leaf epidermal cells transiently expressing MNS1-MNS3-GFP-HDEL or MNS3-MNS1-GFP-HDEL. Scale bars = 10 µm. c Crude protein extracts from leaves of N. benthamiana ∆XF plants transiently expressing MNS1-MNS3-GFP-HDEL or MNS3-MNS1-GFP-HDEL were subjected to Endo H digestion followed by immunoblotting with anti-GFP antibodies. The asterisk marks the mobility shift. Source data are provided as a Source Data file