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Upregulation on the gene expression of gp51, G4, and R3 by PGE2. (A–F) PBMCs from BLV-infected cattle were incubated with PGE2, EP2 agonist, or EP4 agonist, and the gene expression of gp51, G4, and R3 was quantitated by qPCR (A–C, n = 6; D–F, n = 9). Symbols represent mean from single-culture wells of different individuals; pooled data from several experiments were analyzed by the Wilcoxon signed-rank test (A–C) and the Steel–Dwass test (D–F).
