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. 2019 Aug 16;9:11953. doi: 10.1038/s41598-019-48321-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gain of function of Sox17 in Nkx2-5-expressing CPCs. (A) Construction of the BAC-based Nkx2-5^{Sox17-IRES-LacZ} Tg. (B–E) Double whole-mount in situ hybridization for Etv2 (blue) and for either Nkx2-5 (brown in B,D) or LacZ (brown in C,E) in E8.25 Tg(−) or Tg(+) embryos, respectively. (F–J) The heart of a Tg(−) embryo (F) and of Tg(+) embryos with severe (G,H), moderate (I), or mild (J) phenotypes at E9.5. Dashed magenta lines show the outline of the heart tube. (K–M) Immunofluorescence micrographs for the sarcomere myosin heavy chain MYH1 (red) and PECAM1 (green) in the left ventricle of the heart of E9.5 Tg(−) (K) and Tg(+) (L) embryos. (M) The boxed region in L is shown at higher magnification. Blue, DAPI; Arrowheads, cardiomyocytes expressing PECAM1; Scale bars, 100 µm.