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. 2019 Aug 16;9:11953. doi: 10.1038/s41598-019-48321-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cardiac defects associated with mesoderm-specific loss of function for Sox17 in mouse embryos. (A,B) Mesp1^+/+/Sox17^fl/fl (WT) (A) and Mesp1^Cre/+/Sox17^fl/fl (Sox17 KO) (B) embryos at E9.5. Scale bar, 1 mm. (C,D) Immunofluorescence micrographs in the heart of WT (C) and Sox17 KO (D) embryos at E9.75. The boxed regions in the upper panels are shown at higher magnification in the lower panels. Red, PECAM1; Green, TNNT; Blue, DAPI; LA, left atrium; RA, right atrium; LV, left ventricle; RV, right ventricle. Scale bar, 100 µm. (E–G) Reverse transcription and real-time PCR analysis of the relative expression levels for the NOTCH signaling-related genes Dll4 (E), Notch1 (F), and Nrg1 (G) in the heart of WT and Sox17 KO embryos at E8.5 (left panels) and E9.5 (right panels). Nrg1 expression at E8.5 was below the threshold for detection (N.A., not amplified). Only significant P values (Student’s t test) are indicated. Means ± SD.