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. 2019 Aug 16;9:11953. doi: 10.1038/s41598-019-48321-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered gene expression profiles in the endocardium and myocardial cells of mesoderm-specific Sox17 mutant embryos. (A,B) Hierarchical clustering and heat map for 285 (A) and 358 (B) probe sets for differentially expressed genes in the endocardium and myocardial cells, respectively, of WT and Sox17 KO embryos at E8.5. (C,D) Immunofluorescence micrographs for pHH3 (green) and PECAM1 (red) in the heart of WT (C) and KO (D) embryos at E9.5. Blue is DAPI. Arrowheads indicate pHH3⁺ endocardium cells. LV, left ventricle; Scale bars, 50 µm. (E) Proportion of pHH3⁺ cells among PECAM1⁺ cells in the heart of WT and Sox17 KO embryos from E8.5 to E9.5. Means ± SD. Only P values of ≤0.05 (Student’s t test) are indicated.