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. 2019 Jul 26;116(33):16551–16560. doi: 10.1073/pnas.1904885116

Fig. 6.

Fig. 6.

Enhanced lysosomal activity is mediated through leptin-dependent mTORC2 signaling. (A) Immunoblot analysis of mTORC2 pathway in WT versus LEX BMDMs infected with S. Typhimurium for 1 h and 4 h compared with UI (n = 3). (B) Densitometric analysis of phosphorylated Akt to Gapdh (n = 3). (C) Immunoblot analysis of mTORC2 pathway in human macrophages (n = 1). (D) Densitometric analysis of phosphorylated Ndrg1 to Gapdh (n = 3). (E) Bacterial load in Rictor siRNA transfected WT BMDMs compared with control siRNA-transfected cells infected with S. Typhimurium for 24 h. (F) Flow cytometric analysis in Rictor siRNA transfected WT BMDMs compared with control siRNA transfected cells with C12FDG-coated S. Typhimurium 4 h after infection (n = 3). (G) IL-6 expression in supernatants from Rictor siRNA-transfected WT BMDMs compared with control siRNA transfected cells infected with for 24 h. (H) Bacterial load 24 h after infection in WT BMDMs treated with 10 μM Akt-Inhibitor compared with untreated. (I) IL-6 expression in supernatants in 24-h infected WT BMDMs treated with 10 μM Akt-Inhibitor compared with untreated. Data are shown as mean ± SEM and statistical significance calculated using Student t test and represented as *P < 0.05; **P < 0.01; ***P < 0.001.