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. 2019 Jul 26;116(33):16551–16560. doi: 10.1073/pnas.1904885116

Fig. 7.

Fig. 7.

Loss of leptin signaling enhances Phlpp-1-dependent dephosphorylation of Akt to enhance lysosomal function. (A) Immunoblot analysis of Phlpp1 expression in WT versus LEX BMDMs at 1 and 4 h after infection compared with UI (n = 3). (B) Densitometric analysis of Phlpp1 to Gapdh (n = 3). (C) Expression of Phlpp1 and phospho-AKT Ser473 in Phlpp1-depleted LEX BMDMs compared with control cells (n = 3). (D) Bar graph represents flow cytometric measurement of FDG-positive cells in Phlpp1-depleted LEX BMDMs compared with control cells. (E) Bacterial burden in Phlpp1-deficient LEX BMDMs compared with control cells 24 h after infection. (F) IL-6 expression in supernatants of Phlpp1-depleted LEX BMDMs compared with control cells 24 h after infection. Data are shown as mean ± SEM and statistical significance calculated using Student t test and represented as *P < 0.05; **P < 0.01; ***P < 0.001.