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. 2019 Jul 25;116(33):16541–16550. doi: 10.1073/pnas.1903991116

Fig. 1.

Fig. 1.

The cellular kinome is more similar within subtypes than across subtypes. (A) Euclidian hierarchical clustering of 24 lymphoma cell lines from 5 subtypes and primary B cells that were subject to MIB/MS. Data presented are the average of 2 biological replicate MIB/MS runs for each cell line and the average of 5 replicate MIB/MS runs for primary B cells. Gray color indicates a kinase that was not captured in that cell line. (B) A select set of kinases having greater MIB binding in all cell lines relative to primary B cells is graphed. Points within each lymphoma subtype represent a single cell line. Points for primary B cells are from individual mass spectrometry runs. Data presented are the mean ± SEM with significance (ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, degrees of freedom = 24) tested between primary B cells and each NHL subtype. Reported significance is the highest P value of all comparisons between each NHL subtype to the primary B cells. (C–F) A select set of kinases: (C) KS6B1, (D) ATR, (E) LIMK2, and (F) PKN1 displaying MIB-binding bias across subtypes. Each point represents a single cell line. Data presented are the mean ± SEM (ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, degrees of freedom = 19). Refer to Dataset S2 for official gene names of the kinases. LFQ indicates label-free quantification.