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. 2019 Jul 26;116(33):16410–16419. doi: 10.1073/pnas.1904108116

Fig. 5.

Fig. 5.

The lncRNA-CCL2 positively regulates levels of its neighboring CCL2 gene. (A and B) The qRT-PCR data of lncRNA-CCL2 and CCL2 gene expression upon knockdown of lncRNA-CCL2 using (A) siRNA or (B) GapmeR under basal condition and upon stimulation with IL-1β for 2 h in HUVEC. *P < 0.05, paired Student’s t test, (A) n = 3, (B) n = 8. (C) The qRT-PCR data of unspliced pre-CCL2 gene expression upon knockdown of lncRNA-CCL2 using GapmeR under basal condition and upon stimulation with IL-1β for 2 h in HUVEC. *P < 0.05, paired Student’s t test, n = 5. (D and E) qRT-PCR data of lncRNA-CCL2 and CCL2 expression upon knockdown of lncRNA-CCL2 using GapmeR under basal conditions or upon stimulation with IL-1β for 2 h in (D) EC-RF24 and (E) HMEC1. *P < 0.05, paired Student’s t test, (D) n = 3, (E) n = 5. (F) The qRT-PCR data of CCL genes upon knockdown of lncRNA-CCL2 using GapmeR and stimulation with IL-1β for 2 h in HUVEC. *P < 0.05, paired Student’s t test, n = 4; NS, nonsignificant. (G) The qRT-PCR data of inflammatory genes upon knockdown of lncRNA-CCL2 using GapmeR and stimulation with IL-1β for 2 h in HUVEC. *P < 0.05, paired Student’s t test, n = 4.