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. 2019 Jul 26;116(33):16410–16419. doi: 10.1073/pnas.1904108116

Fig. 6.

Fig. 6.

The regulation of CCL2 levels by lncRNA-CCL2 may, in part, be mediated through interaction with RNA-binding proteins. (A) The qRT-PCR data showing the distribution of MT-CO2 (cytoplasmic control), GAPDH (cytoplasmic control), lncRNA-CCL2, and MALAT1 (nuclear control) in the nuclear and cytoplasmic compartments of 2-h IL-1β−stimulated HMEC1, n = 3. (B) Table showing the functional terms associated with the proteins uniquely pulled down or enriched in pull-downs using a biotinylated lncRNA-CCL2 probe in nuclear extracts of IL-1β−stimulated HMEC1. (C) Validation of IGF2BP2 and HNRNPU pull-down with the biotinylated lncRNA-CCL2 probe by Western blot. (D) The qRT-PCR data of IGF2BP2, lncRNA-CCL2, and CCL2 expression upon knockdown of IGF2BP2 using siRNA under basal and 2-h IL-1β−stimulated conditions in HMEC1. *P < 0.05, paired Student’s t test, n = 4. (E) The qRT-PCR data of HNRNPU, lncRNA-CCL2, and CCL2 expression upon knockdown of HNRNPU using siRNA under basal and 2-h IL-1β−stimulated conditions in HMEC1. *P < 0.05, paired Student’s t test, n = 4.