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Confirmation of (A) hn (1000 bp) and (B) f (852 bp) genes cloning by PCR and digestion. In (A) and (B), lane 1 corresponds to the non-recombinant plasmid and lane 2 the positive control. (C) The recombinant plasmids were digested with BamHI /XhoI restriction enzymes, lane 1: pET32a (+) (5900 bp), lane 2: pET-32a (+)/f(852 bp), lane 3: pET-32a (+)/hn (1000 bp), and M: DNA Ladder Mix (Thermo).
