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Primer sequences used in this experiment
| Primer | Sequence (5′-3′) | Restriction site | PCR product (length; bp) |
|---|---|---|---|
| HNf | TCTAAGGATCCACACCACTGGGTTGCGACA | BamHI | hn (999) |
| HNr | CTTGCTCTCGAGTTATTCTGTCTTCAGGAGTGCCA | XhoI | |
| Ff | TGTTCGGATCCAAAAACATCTCCATCCAAGA | BamHI | f (852) |
| Fr | CTATTGCTCGAGTTATGAATCGTACAGGATTGGGT | XhoI |
