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. 2019 Jul 31;116(33):16326–16331. doi: 10.1073/pnas.1820683116

Fig. 2.

Fig. 2.

Diffusive exchange of phase-separated Rv17471–310 slows on phosphorylation. (A) FRAP measurements of exchange at room temperature in droplets formed by unmodified OG-Rv17471–310 (>250 μM; Top) or phosphorylated OG-Rv17471–310 (50 μM treated with 0.5 μM PknF and 100 μM ATP; Bottom). The bleached sector at t = 0 is indicated by an arrow, followed by images obtained at 2 subsequent time points. (Scale bars: 5 μm.) Although fluorescence recovery is likely dominated by diffusive exchange within the droplet, exchange with surrounding liquid phase is also possible. (B) FRAP recovery was quantified by fitting the average normalized intensity (solid dots with SD bars) to a single exponential function (black lines) for 3 different droplets in 1 sample of either unmodified or phosphorylated Rv17471–310.