Fig. 1.

SRY expression is up-regulated in experimental models of PD. (A and B) Time courses of nigral Sry, Gadd45g, and Th mRNA expression (as fold change of the intact side relative to Tbp1) at 0 to 21 d following a single intranigral 6-OHDA (A) or rotenone (B) injection in male rats (n = 5/group). #P < 0.05; ##P < 0.01; ###P < 0.001 vs. d 0, 1-way ANOVA. The dashed line represents baseline levels. (C and D) Time course of SRY or GADD45G mRNA expression (as fold change of 0 h relative to β2M) at 0 to 72 h after treatment with 6-OHDA (C) or rotenone (D) in human M17 cells (n = 5/group). #P < 0.05, ##P < 0.01 vs. 0 h, 1-way ANOVA. (E) Effect of vehicle, 6-OHDA, or rotenone treatment on SRY protein (green) or DAPI (blue) expression at 24 h posttreatment in human M17 cells (n = 4/group). *P < 0.05 vs. vehicle, unpaired t test. (Scale bar: 10 µm.)