Table 2.
Primers used for real-time PCR analysisa
| Gene name | Primer sequence (5′ to 3´) | Product size, bp | Efficiency, % | Accession number |
|---|---|---|---|---|
| CAT-1 | F: ATGTAGGTTGGGATGGAGCC | 280 | 113.556 | XM_015277949.1 |
| R: AACGAGTAAGCCAGGAGGGT | ||||
| CAT-2 | F: CAAGTCTTCTCGGCTCTAT | 105 | 105.602 | XM_015285435.1 |
| R: GTGCCTGCCTCTTACTCA | ||||
| CAT-3 | F: CAAGACTGGCTCTGCCTACC | 236 | 106.623 | XM_015278426.1 |
| R: GGATCAACGCAAAGAAGTCC | ||||
| iNOS | F: TGGGTGGAAGCCGAAATA | 241 | 103.087 | NM_204961.1 |
| R: GTACCAGCCGTTGAAAGGAC | ||||
| ARG2 | F: GCCAACTGTACGACTTTGGAG | 150 | 90.430 | NM_001199704.1 |
| R: AGCTGTGTCCAGCAGCTACC | ||||
| ADC | F: CCCAGTTTGAGGAGATTGCT | 122 | 108.230 | XM_004947849.2 |
| R: AAAGGTGAAGGCTGAGGTGA | ||||
| AGAT | F: TCGTCAAGAGGCCTGATCCA | 140 | 111.489 | XM_015291975.1 |
| R: CCAAGCCATAGGCGCTTCAA | ||||
| IL-1β | F: ACTGGGCATCAAGGGCTA | 131 | 104.419 | XM_015297469.1 |
| R: GGTAGAAGATGAAGCGGGTC | ||||
| IL-6 | F: CGCCCAGAAATCCCTCCTC | 152 | 101.974 | XM_015281283.1 |
| R: AGGCACTGAAACTCCTGGTC | ||||
| IL-8 | F: ATGAACGGCAAGCTTGGAGCTG | 233 | 119.885 | NM_205498.1 |
| R: TCCAAGCACACCTCTCTTCCATCC | ||||
| IFN-γ | F: AGCTGACGGTGGACCTATTATT | 259 | 108.556 | NM_205149.1 |
| R: GGCTTTGCGCTGGATTC | ||||
| IL-10 | F: CGCTGTCACCGCTTCTTCA | 88 | 98.435 | NM_001004414.2 |
| R: TCCCGTTCTCATCCATCTTCTC | ||||
| TGF-β3 | F: CATCGAGCTCTTCCAGATCC | 112 | 102.986 | NM_205454.1 |
| R: GACATCGAAGGACAGCCACT | ||||
| JAK1 | F: TGCACCGTGACTTAGCAGCAAG | 168 | 114.087 | XM_015290965.1 |
| R: TCTGAATCAAGCATTCTGGAGCATACC | ||||
| JAK2 | F: TCGCTATGGCATTATTCG | 197 | 110.029 | XM_015280061.1 |
| R: GTGGGGTTTGGTCCTTTT | ||||
| JAK3 | F: GCATCCGCCGCCGTGTTG | 108 | 96.164 | NM_204996.3 |
| R: AGCACCGCAGCCTCTCCAG | ||||
| STAT1 | F: TAAAGAGGGAGCAATCAC | 112 | 109.954 | XM_015289392.1 |
| R: ATCAGGGAAAGTAACAGC | ||||
| STAT6 | F: GCAACCTCTACCCCAACA | 127 | 101.256 | XM_015274736.1 |
| R: TCCCTTTCGCTTTCCACT | ||||
| GAPDH | F: TGCTGCCCAGAACATCATCC | 142 | 98.200 | NM_204305.1 |
| R: ACGGCAGGTCAGGTCAACAA | ||||
aAbbreviations: CAT Cationic amino acid transporter, iNOS inducible nitric oxide synthase, ARG2 Arginase 2, ADC Arginine decarboxylase, AGAT Arginine: glycine amidinotransferase, IFN-γ Interferon-γ, TGF-β3 Transforming growth factor-β3, JAK Janus kinase, STAT Signal transducer and activator of transcription, GAPDH Glyceraldehyde-3-phosphate dehydrogenase, F Forward, R Reverse