Fig. 4.

Restoration of 15-LOX1 and 15-LOX2 activities inhibited the effects of PM2.5 and NNK. a Restoration of 15-LOX1 and 15-LOX2 activities inhibited the effects of PM2.5 or NNK on vimentin expression. After Bet1A cells were treated with PM2.5 or NNK for 28 days, the cells were transfected with 15-LOX1, 15- LOX2 or vector plasmid DNA respectively for 24 h. 15-LOX1, 15-LOX2 and vimentin were determined. The quantification of protein was carried out by densitometry analysis and expressed as mean ± SE. The relative intensity of protein bands was summarized by column figure (n = 3, **p < 0.01when compared each PM2.5- or NNK-treated condition with the control cells; and ^##P < 0.01 when compared the condition of cells transfected with 15-LOX1/15-LOX2 and treated with PM2.5 or NNK with the condition of cells transfected with pcDNA3.1 and treated with PM2.5 or NNK respectively). b Restoration of 15-LOX1 and 15-LOX2 activities inhibited the effects of PM2.5 or NNK on cell migration. Bet1A cells treated with PM2.5 or NNK for 28 days were transfected with 15-LOX1, 15- LOX2 or vector plasmid DNA respectively for 24 h. Wound-healing assay were performed