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. 2019 Aug 16;20:656. doi: 10.1186/s12864-019-6028-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The features of AGO18b-bound sRNAs and total sRNAs by cRIP-seq and sRNA-seq, respectively. a Western blot experiment of AGO18b and IgG for W22-ref samples (top) and ago18b::mum samples (bottom). b Pie chart showing the average percentages of different sRNAs in RIP-seq libraries from two independent biological replicates. c Line plot showing the length distribution of sequenced sRNA reads in sRNA-seq libraries and AGO18b-bound sRNA libraries. The 21-nt sRNAs were preferred bound by AGO18b in cRIP-seq samples. d Bar plot showing the ratio between 21-nt and 24-nt sRNAs in the sRNA libraries and AGO18b-bound sRNA libraries. e Bar plot showing the percentages of 21-nt and 24-nt phasiRNAs in AGO18b-bound sRNA libraries