Fig. 7.

The predicted RNase active site of smut_2965 is required for its cell-death triggering ability. a The mutant proteins smut_2965–60, smut_2965–79, smut_2965–96, and smut_2965–112 lost the ability to induce cell-death, while smut_5844–68 and smut_5844–174 induced cell-death in Nicotiana benthamiana leaves. GFP refers to the PMDC32 vector containing the green fluorescent protein (GFP) gene, the same labeling is used for 2965, 5844, 2965∆60, 2965∆79, 2965∆96, 2965∆112, 5844∆68, and 5844∆147. Numbers, e.g., 0/35, indicate that 0 of 35 infiltrated leaves exhibiting cell-death or mottling phenotypes. b Protein expression of the smut_2965 and smut_5844 mutant proteins in the infiltrated leaves was detected by western blotting. M:marker; b1–6: smut_2965∆60 (13.74 kDa), smut_2965∆79 (13.74 kDa), smut_2965∆96 (13.74 kDa), smut_2965∆112 (13.74 kDa), smut_5844∆68 (24.72 kDa), and smut_5844∆147 (24.72 kDa)