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SiRNA strategy suppresses the PYCR1 expression successfully and retards cell viability in Ketr-3 cells. (A and B) The effective rates were detected by qRT-PCR analysis and western blot. (C) The fold change of western blot results. (D) Cell proliferation was measured using CCK-8 assay. (E) Colony formation was measured and (F) the number of clone was counted. The data are expressed as mean ± SD in triplicates. (*P < 0.05, **P < 0.01)
