Table 3.

Primers used to amplify P4ha1 and EGFP-KDEL and mutate P4ha1

Gene name sequence

P4ha1  Sense: 5′ATTTGCGGCCGCCACCATGATCTGGGTTGTTTTAATGA-3′  Antisense: 5′-CTAGCTAGCTTCCAATTCTGACAGGGTG-3′ HA-tagged-P4ha1  Sense: 5′-CGTAAAAAACCCTGGATGAGCGTAATCTGGAACATCGTATGGGT AGGCTAAAGACTGAGGAAG-3′  Antisense: 5′-CTTCCTCAGTCTTTAGCCTACCCATACGATGTTCCAGATTACGCT CATCCAGGGTTTTTTACG-3′ P4ha1-N113Q  Sense: 5′-ACTGCCTCTGAATGGTTAGTTGAGAGATGAAGCCATCCGAC-3′  Antisense: 5′-GTCGGATGGCTTCATCTCTCAACTAACCATTCAGAGGCAGT-3′ P4ha1-N259Q  Sense: 5′-GGTCGCCCGACGCTGACTTTTGGGCATCTTTTTCTTTACTCA-3′  Antisense: 5′-TGAGTAAAGAAAAAGATGCCCAAAAGTCAGCGTCGGGCGACC-3′ Signal peptide of Calreticulin  Sense: 5′-ATTTGCGGCCGCCACCATGCTCCTTTCGGTGCCG-3′  Antisense: 5′-CAGCTCCTCGCCCTTGCTCACTGCGGCGGCCAGGCCGAGGA-3′ Egfp  Sense: 5′-TCCTCGGCCTGGCCGCCGCAGTGAGCAAGGGCGAGGAGCTG-3′  Antisense: 5′-GCTCTAGATTACAGCTCATCCTTGTACAGCTCGTCCATGCC-3′