Fig. 6.

Activation of CD11b⁺ and CD45⁺-microglia in the ganglion cell layer of the retina by loss of Ranbp2 in RGNs. Confocal images of retinal flat mounts (ganglion neurons facing up) of +/+ and −/− mice immunostained for CD11b (A) and CD45 (B) on d10. Compared to +/+ (A, a–d), there is prominent activation of CD11b⁺-microglial cells, whose proliferative processes surround YFP⁺-RGNs of −/− mice (A, e–h). Compared to +/+ (B, a–d), there is also prominent activation of CD45⁺-microglial cells, whose proliferative processes surround YFP⁺-RGNs of −/− mice (B, e–h). Figures a′–h′ in A and B represent lateral views of z-stacks of a–h and collapsed from a 25 µm thick-stack of 13 images captured 2 µm apart. Scale bars 50 µm. d10 day 10 post-tamoxifen administration, −/− SLICK-H::Ranbp2^flox/flox, +/+ SLICK-H::Ranbp2^+/+