Ligation the left anterior descending artery.
A. Prolene ligature is passed underneath the left anterior descending artery
B. The left anterior descending artery is ligated with a prolene suture and myocardial infarction is created. The discoloration of the surrounding tissue and electrocardiography confirms the creation of an infarct.
C. Rat left ventricle was stained with Masson’s trichrome and Weigert’s iron hematoxylin. Blue area indicates left ventricular scar 4 weeks after myocardial infarction.