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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Jun 17;133:174–187. doi: 10.1016/j.yjmcc.2019.06.006

Figure 4. A fraction of single nuclear diploid cardiomyocytes is further increased in de-AAC compared to AAC at postoperative day 21.

Figure 4.

A. Representative images of isolated EdU+ cardiomyocytes (left panels, scale bar = 50 μm). Percentage of thymidine analog positive single nuclear cardiomyocyte is increased in de-AAC compared to that in AAC at day 21 (*p <0.05 vs sham, †p <0.05 vs AAC at day 21), which is restored to AAC level at day 49 (‡p <0.05 vs sham at day49). B. Cardiomyocytes ploidy analysis at day 21, following AAC and de-AAC, respectively. Y chromosomes are detected by FISH. Each single spot indicates a single Y chromosome. One spot in single nuclei indicates diploid cell (upper left panel for example, scale bar = 10 μm). Percentage of single nuclear diploid cardiomyocyte is increased in de-AAC compared to sham (*p <0.05 vs sham), while there is no difference in multi nuclear cardiomyocyte.