Figure 5.

Ca²⁺ Responses and Glutamate Inputs in glc-3 Mutants

(A) Averaged glutamate inputs (blue) and Ca²⁺ responses (red) to odor. The shaded region indicates IAA stimulation, and the shadows around the solid lines indicate the SEM (N = 12).

(B) Glutamate intensities before (pre-addition) and after (post-addition) odor stimulation onset (left), and average intensities before (pre-removal) and after (post-removal) odor stimulation removal (right). The statistical metrics are as follows: left, p = 0.0030; right, p = 0.0018; paired t test (N = 12). **p < 0.01.

(C) Averaged glutamate responses coincident with Ca²⁺ spikes. Time 0 indicates the onset of Ca²⁺ spikes. All glutamate responses were normalized to zero at time 0. The purple, blue, and green lines indicate the responses under the no odor, early odor, and later odor conditions, respectively. Shadows indicate the SEM (N = 12, no odor, n = 15; early odor, n = 6; later odor, n = 20).

(D) Glutamate input before the Ca²⁺ spike and at spike onset. “Pre” indicates the average intensity from −3 to −2 s in (C), and “onset” indicates the average intensity from −0.5 to +0.5 s. The statistical metrics are as follows: no odor, p = 0.0012; early odor, p = 0.65; later odor, p = 0.89; paired t test (N = 12, no odor, n = 15; early odor, n = 6; later odor, n = 20). **p < 0.01.

(E) Averaged glutamate decreases with (filled boxes) and without (hollow boxes) Ca²⁺ spikes. Box plots indicate the median (center line), quartiles (boxes), and range (whiskers). The statistical metrics are as follows: no odor, p = 0.0027; later odor, p = 0.58; Welch's t test (N = 12, no odor, spike, n = 15; no odor, no spike, n = 114; later odor, spike, n = 20; later odor, no spike, n = 82). **p < 0.01.