Figure 5.

Plasma amino acids and trace elements during the first renal replacement therapy (RRT) session (RRT1) and second RRT session (RRT2). Plasma was sampled before (base) and at the end (end) of each RRT session. Micronutrients and amino acids were measured in spot samples of plasma as described in the Methods. Data (mean ± SE) are presented corrected (i.e., included as covariates in the statistical model) for dose-of-dialysis (plasma urea reduction ratio) with or without log₁₀ transformation if necessary. Analysis was by repeated-measures analysis of variance or mixed-effect models (Genstat v18; VSNi, Rothampsted, UK), as appropriate, with RRT mode, time, and session (first or second) as fixed effects. Patient ID was included as a nested random effect. Statistical significance was accepted at P ≤ 0.002 (adjusted for the number of comparisons). The data emphasize that our broad assumptions on the change in plasma micronutrients during RRT1 are largely applicable during RRT2, despite moderate depletion of micronutrient status. (a,b) Change in total plasma amino acid concentration for RRT-1 and RRT-2, respectively; (c,d) data for plasma glutamine; (e,f) data for plasma total trace element concentration. CVVH, continuous veno-venous hemofiltration; IHD, intermittent hemodialysis; SLEDf, sustained low-efficiency diafiltration.