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. Author manuscript; available in PMC: 2019 Aug 18.
Published in final edited form as: Nat Cell Biol. 2018 Nov 5;20(12):1410–1420. doi: 10.1038/s41556-018-0221-1

Figure 4. ncBAF subunit domains underlie complex-specific synthetic lethalities.

Figure 4.

a. Schematic for tiled gRNA CRISPR-Cas9 screening performed across mSWI/SNF subunit genes in SYO-1 SS cells.

b. Tiled CRISPR screening for the GLTSCR1 gene in SYO-1 cells.

c,d. Tiled CRISPR screening for the BRD9 gene (c) and BRD7 gene (d) in SYO-1 cells.

e. Alignment of GLTSCR1 amino acid sequences across species. GLTSCR domain is highlighted.

f. Alignment of amino acid sequences for BRD9 and BRD7 across species. Bromodomain and DUF3512 are highlighted.

g. (Top) Construct design for GLTSCR domain experiments in HEK-293T cells. (Bottom) Immunoprecipitation of V5-tagged constructs followed by immunoblot (n=2 biologically independent experiments). See also Supplementary Figure 7f.

h. (Left) Construct design for C-terminal swap experiments for BRD9 and BRD7 in HEK-293T cells (BD = bromodomain). (Right) Immunoprecipitation of BRD9, BRD7, and BRD7(B9C) and BRD9(B7C) C-terminal swap variants followed by immunoblot in HEK-293T cells (n=2 biologically independent experiments). See also Supplementary Figure 7g.