Figure 5 –

Intra-animal comparison of tree shrew cone density from ROIs captured at the same eccentricity relative the optic nerve in vivo and ex vivo. Split detector AOSLO image (A) and whole-mount (WM) immunofluorescent image with L/M-opsin (green) and S-opsin (blue) (B) both 2.3 mm nasal from the ONH of TS2. (A & B) In this example, the in vivo density from AOSLO (33,491 cones/mm²) is lower than the ex vivo density from the whole-mounted retina (41,275 cones/mm²). Scale bars = 20 μm. AOSLO (AO) cone densities were significantly lower compared to WM cone densities from locations equidistant from the ONH for both TS1 and TS2 (C). **P < 0.01, Wilcoxon match-pairs signed ranks test.