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. Author manuscript; available in PMC: 2019 Aug 18.
Published in final edited form as: Pediatr Res. 2019 Feb 18:10.1038/s41390-019-0340-9. doi: 10.1038/s41390-019-0340-9

Figure 5. Single cell RNAseq characterization of differentiated PHLE cells.

Figure 5.

PHLE cells differentiated at ALI (n=168, from 4 donors) were captured on the C1 platform and subjected to single cell RNA sequencing. Cells passing quality filtering (n=148) were assigned to a lineage based upon expression of single markers (FOXJ1, KRT5, MUC5B and SFTPB) using Monocle. Gene expression diversity among the cells is displayed in 2D tSNE space where each plot element represents a cell. Four separate plots are presented, one for each lineage marker. Plot elements are colored to represent individual cell lineages, with the size of the dot representing the relative enrichment in expression for that lineage marker. Olive dots represent cilated lineage (FOXJ1), green represents basal lineage (KRT5), turquoise represents goblet lineage (MUC5B), blue represents secretory lineage (SFTPB), red dots represent ambiguous cell lineage (expression of multiple markers) and pink represents unknown lineage.