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. Author manuscript; available in PMC: 2020 Jan 12.
Published in final edited form as: J Mol Biol. 2019 May 17;431(15):2821–2834. doi: 10.1016/j.jmb.2019.04.048

Fig. 4. Autophagy prevalent conditions reveal presence of a subcomplex of exocyst comprising of subunits that are required for autophagy.

Fig. 4

(a) Sec8-GFP cells were grown in rich medium (YPD) and starved for 4 h. Clarified supernatant (cytosol) from YPD or SD-N grown cells were prepared and subjected to size exclusion chromatography using Superose 6 high load 10/300GL column. Fractions were collected and analyzed by Western blotting using anti-GFP antibody. L.E., lower exposures; H.E., higher exposures. (b) Intensities of bands from panel A were quantitated and plotted against fractions. Peak in the green area represents higher-molecular-weight exocyst complex associated with secretory function, while peak in the pink area represents starvation-specific exocyst subcomplex. (c) Strains expressing exocyst subunits tagged with GFP were starved for 4 h and processed as in panel a. Fractions were analyzed by Western blotting. Intensity of bands from these Western blots is plotted in panel d.