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. Author manuscript; available in PMC: 2020 Jan 12.
Published in final edited form as: J Mol Biol. 2019 May 17;431(15):2821–2834. doi: 10.1016/j.jmb.2019.04.048

Table 1. List of strains and plasmids used in this study.

Sr. No. Strain/plasmid name Genotype Source/Reference
1 sSUN28 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
2 sSUN29 sec5–24::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
3 sSUN30 sec6–4::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
4 sSUN31 sec8–6::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
5 sSUN32 sec8–9::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
6 sSUN33 sec10–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
7 sSUN34 sec15–1::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
8 sSUN35 exo70–38::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
9 sSUN37 Exo84–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; POT1::POT1-GFP-HIS This study
10 sSUN53 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
11 sSUN54 sec5–24::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
12 sSUN55 sec6–4::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
13 sSUN56 sec8–6::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
14 sSUN57 sec8–9::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
15 sSUN58 sec10–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
16 sSUN59 sec15–1::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
17 sSUN60 exo70–38::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
18 sSUN62 exo84–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
19 sSUN99 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::KanMX4 Euroscarf
20 sSUN100 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; YPT7::KanMX4 Euroscarf
21 sSUN105 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; VAM3::Hph; VAM3ts (p415) This study
22 sSUN1 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; [43]
23 sSUN107 sec3–2::KanR; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; VAM3::Hph; VAM3ts (p415) This study
24 sSUN108 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; GFP-Atg8:: URA 3 [pGFP-Atg8 in pRS316] This study
25 sGRB20 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; pGFP/N-Aut9 [44]
26 sGRB21 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::KanMX4; pGFP/N-Aut9 [44]
27 sSUN63 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; pGFP/N-Aut9 This study
28 sSUN101 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::Hph; pGFP/N-Aut9 This study
29 sGRB35 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; pGFP/N-Aut9; pJH1M [44]
30 sSUN102 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; pGFP/N-Aut9; pJH1 This study
31 sSUN109 exo70–38::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; pGFP/N-Aut9 This study
32 sSUN110 exo70–38::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::Hph; pGFP/N-Aut9 This study
33 sSUN111 exo70–38::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; pGFP/N-Aut9; pJH1 This study
34 sSUN113 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::Hph; ATG27::KanMX; pGFP/N-Aut9 This study
35 sSUN73 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC3::SEC3-GFP-HIS [45]
36 sSUN74 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC5::SEC5-GFP-HIS [45]
37 sSUN75 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC6::SEC6-GFP-HIS [45]
38 sSUN76 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC8::SEC8-GFP-HIS [45]
39 sSUN77 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC10::SEC10-GFP-HIS [45]
40 sSUN78 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC15::SEC15-GFP-HIS [45]
41 sSUN79 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; EXO70::EXO70-GFP-HIS [45]
42 sSUN80 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; EXO84::EXO84-GFP-HIS [45]
43 sSUN103 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC3::SEC3-GFP-HIS; pSUN5 This study
44 sSUN104 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC3::SEC3-GFP-HIS; ATG1::Hph; pSUN5 This study
45 sSUN114 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; EXO70::EXO70-GFP-HIS; pSUN5 This study
46 sSUN115 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; EXO70::EXO70-GFP-HIS; ATG1::Hph; pSUN5 This study
47 sSUN116 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC15::SEC15-GFP-HIS; pSUN5 This study
48 sSUN117 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC15::SEC15-GFP-HIS; ATG1::Hph; pSUN5 This study
49 sSUN81 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC3::SEC3-GFP-HIS; 2XmCherry-Atg8::URA3 [2XmCherry-Atg8 in pRS316] This study
50 sSUN118 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC3::SEC3-GFP-HIS; YPT7::Hph; 2XmCherry-Atg8::URA3 [2XmCherry-Atg8 in pRS316] This study
51 sSUN121 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC15::SEC15-GFP-HIS; 2XmCherry-Atg8::URA3 [2XmCherry-Atg8 in pRS316] This study
52 sSUN122 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; SEC15::SEC15-GFP-HIS; YPT7::Hph; 2XmCherry-Atg8::URA3 [2XmCherry-Atg8 in pRS316] This study
53 sSUN119 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; EXO70::EXO70-GFP-HIS; 2XmCherry-Atg8::URA3 [2XmCherry-Atg8 in pRS316] This study
54 sSUN120 MAT a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; EXO70::EXO70-GFP-HIS; YPT7::Hph; 2XmCherry-Atg8::URA3 [2XmCherry-Atg8 in pRS316] This study
55 sSUN123 BY4741; Mat a; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::KanMX4; pGFP/N-Aut9; pSUN8 This study
56 sSUN124 sec3–2::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::Hph; pGFP/N-Aut9;pSUN8 This study
57 sSUN125 exo70–38::KanR; his3Δ1 leu2Δ0 ura3Δ0 met15Δ0; ATG1::Hph; pGFP/N-Aut9;pSUN8 This study
58 GFP-Atg8 in pRS316 Prof. Yoshinori Ohsumi
59 2XmCherry-Atg8 in pRS316 Prof. Yoshinori Ohsumi
60 pSUN5 [44]
61 pJH1 Prof. Michael Thumm
62 pGFP-N/AUT9 Prof. Michael Thumm
63 Vam3ts in pRS316 Prof. Rosine Haguenauer-Tsapis [46]
64 pSUN8 Atg1ts allele PCR amplified from pATG1ts 415 (4) and subcloned into pRS315 This study