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. 2019 Aug 14;14:6519–6538. doi: 10.2147/IJN.S215250

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© 2019 Zhao et al.

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Intracellular trafficking of FPCPNDs in 4T1 cells at different time intervals, scale bar =20 μm. The fluorescence signals were examined by LSCM with three channels: blue fluorescence from nuclei stained with Hoechst 33258, green fluorescence from FITC-labeled FPCPNDs and red fluorescence from the lysotracker stained endo-lysosomes (colocalization indicated by yellow fluorescence).

Abbreviations: FPCPNDs, FA-PEG-CCTS/PEI/NLS/pDNA; PND, PEI/NLS/pDNA; FA, folate acid; PEG, polyethylene glycol; CCTS, carboxylated chitosan; PEI, polyethyleneimine; NLS, nuclear localization sequences; FITC, fluorescein isothiocyanate; LSCM, laser scan confocal microscope.