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. 2019 Jun 19;47(14):7321–7332. doi: 10.1093/nar/gkz492

Figure 3.

Figure 3.

Effects of chemical modifications in the cRNA-strand on miRNA silencing and patterns of serum molecule binding. (A) qRT-PCR analysis of miR-122 in liver tissues from mice treated with 24 nmol/kg injections of the parent antimiR or HDO-antimiRs with varying chemical modifications at both ends of the cRNA-strand; mean values ± SEM (n = 5); *P < 0.05; multiple comparisons were performed using one-way ANOVA with Bonferroni's test. (B) qRT-PCR analysis of miR-122 expression in Huh-7 cells at 24 h after treatment with increasing concentrations of antimiR or HDO-antimiR without serum (n = 4). (C) EMSA after incubation with increasing concentrations of mouse serum show serum protein binding by Cy3–labeled parent antimiR or HDO-antimiR (2′OMe6 PS6) with six 2′OMe and six PS modifications at both ends of the cRNA-strand; positions of bands corresponding to complexes of serum molecules with parent antimiR or HDO-antimiR are indicated by arrow heads and arrows, respectively. (D) EMSA analysis of binding patterns between the Cy3-labeled antimiRs and 7.5 μl serum are presented as in Figure 3A.